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TMA-containing compound assay Trimethylamine-containing compounds were extracted from 50 l of plasma, 50 l of whole liver homogenate or from 100 l of whole brain homogenate (without the cerebellum and olfactory bulbs) by adding 10 volumes of ACN and spiking the samples with 5 l of IS mix containing 12 M d9-TMAO (CIL), 649 nM d9-TMA (CDN Isotopes), 6 M d3-L-carnitine (CIL), 329 nM d9-crotonobetaine (see Koeth et al., 2014) and 5 M d9-TML (Santa Cruz Biotechnology) at the beginning of the extraction

rabbit polyclonal antibody to OGG-1 (1:500 IgG isotype for WB application) (Elabscience ct n E-AB-60300)

Side Effects and Safety Considerations The most common side effects in trials were gastrointestinal including nausea, vomiting, and diarrhoea and were dose-dependent

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