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Under heat stress, sHSPs (such as HSPB1/HSP27) can be phosphorylated and activated via the p38/MAPK-MK2 signaling pathway (Gallagher et al., 2024), thereby initiating autophagy to clear damaged organelles via the lysosomal pathway (Cui et al., 2025)
ELISA assays complement the HPLC analysis by measuring concentrations of melatonin and neurotrophic factors, utilizing kits from Sangon Biotechnology Company (Shanghai, China) following strict protocols to ensure reliability
It is also more convenient and cost effective than most wet foods
Clinical trials have shown that FPG and HOMA-IR decreased in the DASH diet group, which may be related to the improvement of -cell function by magnesium (27, 29)
The morning when spermatozoa were found was designated as gestational day 1 (GD 1)
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