It upregulates genes involved in collagen synthesis (COL1A1, COL3A1, COL5A1), extracellular matrix remodeling (decorin, fibromodulin), and antioxidant defense (superoxide dismutase, glutathione peroxidase) while simultaneously suppressing genes associated with inflammation (IL-6, TNF-alpha), fibrinogen production, and tissue destruction (several MMPs)
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The cells were lysed by ultrasound for 20 min at 4 C, followed by centrifugation at 12,000 rpm for 15 min to collect the supernatant
Use aseptic technique, calibrated pipettes or syringes, and validated diluent choices for the assay system