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75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)
No aspiration needed: Aspiration (pulling back on plunger) is not necessary for subcutaneous injections, as there are no large blood vessels in subcutaneous tissue [11]
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495 Significant progress has been made in DMD therapeutic strategies, including NADs
