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Using these enzyme preparations with a defined GSH concentration (20 M) in the established two-step in vitro reactions, we found that the extent of conversion of the ultrafiltered flavan-3,3,4-triol ( 4 ) depended on the applied concentration of the purified Pt GSTF8, confirming that this enzyme is required and sufficient for the observed conversion to cyanidin (Extended Data Fig

3 : :

Moreover, the complexity of the cell composition of this tissue and the elevated oxygen levels corroborate to elevated capacity of the CNS in ROS production

However, the mechanisms of heme transport and insertion into respiratory chain complexes remain unclear

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