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hGLP-1, pGLP-1, eGLP-1 and Ex-4 (7 M) were incubated with purified human serum in 37 C incubator in the presence/absence of 100 M of DPP-4 inhibitor P32/98 (Santa Cruz Biotech), and aliquots of the reaction solutions were extracted after 0, 3, 6, 9 and 11 h incubation respectively, when TFA (0.1%) was added to terminate the reactions

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