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The cells were routinely maintained in DMEM medium supplemented with 20% (v/v) FBS, 1% penicillin-streptomycin, 1% glutamine and 1% non-essential amino acids in a humidified 5% CO2 incubator (model 3110
Some patients may experience: Mild injection site reactions (redness, slight swelling) Temporary drowsiness (related to increased melatonin production) Mild headache in the first few days Serious adverse effects are rare in published literature
(PubMed) McEvoy CT, Milner KF, Scherman AJ, et al
A byproduct of this metabolically intensive process is creation of free radicals
Equal amounts of protein (20 g) were separated by 8%~15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride membranes (Millipore, Cat.no
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