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This study had several notable strengths
Vigorous shaking creates mechanical stress that promotes aggregation, the clumping of peptide molecules that reduces potency
Joe Rogan's famous testimonial involved exactly this scenario
StatPearls, updated 2024 2 Wylon K, Drozdenko G, Krannich A, et al
Molecular modelling and mutagenesis studies indicate that agonist positive allosteric modulators target the same general region, but in a distinct sub-pocket at the interface between helices V and VI, which may facilitate the formation of an intracellular binding site that enhances G-protein coupling
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