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Cells were washed with PBS and imaged in 11 mM glucose KRBH buffer using a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices) with a 20 air objective and 488 nm laser, with images taken every 6 seconds, on a 37 C stage
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Ipamorelin is a selective growth hormone secretagogue that stimulates the release of GH from the pituitary gland in a pulsatile manner
DIO mice received 1, 5, 10 or 25 g per l per mouse of mGIPR-Ab/P3 or 25 g per l per mouse of IgG1 control injected ICV every 4 days for five treatments, with body weight and food intake monitored for 44 days
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